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Activity Measured by its ability to induce IL6
secretion by NHDF human normal dermal fibroblasts.
The ED50 for this effect is typically 26
ng/mL.
Measured by its ability to induce IL6
secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811.
The ED50 for this effect is typically 1.57.5
ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >97%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCI.
Shipping The product is shipped with polar packs. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
Interleukin 17 （also known as CTLA8）
is a T cellexpressed
pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of
herpesvirus Saimiri. cDNA clones encoding IL17
have been isolated from activated rat, mouse and human T cells. Human IL17
cDNA encodes a 155 amino acid （aa）
residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL17
containing one potential Nlinked
glycosylation site. Both recombinant and natural IL17
have been shown to exist as disulfidelinked
homodimers. At the amino acid level, human IL17
shows 72% and
63% sequence identity with herpesvirus and rat IL17,
respectively. An IL17
specific mouse cell surface receptor （IL17
R） has been cloned. While the expression of
IL17
mRNA is restricted to activated T cells, the expression of mIL17
R mRNA has been detected in virtually all cells and tissues tested. IL17
exhibits multiple
biological activities on a variety of cells including the induction of IL6
and IL8
production in fibroblasts, the enhancement of surface expression of ICAM1
in
fibroblasts, activation of NFκB
and costimulation of T cell proliferation. The nonglycosylated,
E. coliexpressed
rhIL17
has been shown to be active on human as
well as mouse cell lines.
References:
1. Yao, Z. et al. （1995） J. Immunol. 155:5483.
2. Yao, Z. et al. （1995） Immunity 3:811.
3. Rouvier, E. et al. （1993） J. Immunol. 150:5445

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